The relationship between velocity and the substrate concentration is illustrated by an asymptotic curve.

Michaelis-Menten Curve

The equation representing this curve is called the Michaelis-Menten equation.

Michaelis-Menten equation

Where V0 = initial velocity, and [S] = concentration of substrate.

In the asymptotic curve, the line approaches but never reaches Vmax. Therefore, it is difficult to calculate. Hence, for more convenience and ease of calculation, the reciprocal of this equation is used to create a straight-line graph called the Lineweaver-Burk Plot.

Lineweaver Burk Plot

The Lineweaver Burk Plot can be used to differentiate between the three different types of reversible inhibition, i.e. competitive, uncompetitive and noncompetitive inhibition.

Competitive Inhibition

Competitive Inhibition

Uncompetitive Inhibition

Uncompetitive Inhibition

Noncompetitive Inhibition

Noncompetitive Inhibition

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Courtney Simons
Courtney Simons
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Courtney Simons is a food science professor. He holds a BS degree in food science and a Ph.D. in cereal science from North Dakota State University.